BRD7 Polyclonal antibody

• Featured Product
• KD/KO Validated

BRD7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 51009-2-AP

Synonyms

75 kDa bromodomain protein, BP75, BRD7, bromodomain containing 7, CELTIX1, NAG4, Protein CELTIX 1

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Validation Data Gallery

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  WB analysis of HeLa using 51009-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 51009-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51009-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 51009-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 51009-2-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 51009-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 51009-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 51009-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51009-2-AP (BRD7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse cerebellum tissue, mouse lung tissue, L02 cells, rat brain tissue, SH-SY5Y cells, HeLa cells, Jurkat cells, MDA-MB-231 cells, PC-3 cells, SH-5Y5Y cells
• Positive IHC detected in: human colon cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 25 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Product Information

51009-2-AP targets BRD7 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRD7 fusion protein Ag3911
• Full Name: bromodomain containing 7
• Calculated Molecular Weight: 651 aa, 74 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: BC050728
• Gene Symbol: BRD7
• Gene ID (NCBI): 29117
• RRID: AB_2259226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.

Publications

Species	Application	Title
human	WB	Cancer Discov; A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.; Authors - Jinxiu Li
		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
mouse	IF	Nat Commun; SS18 regulates pluripotent-somatic transition through phase separation.; Authors - Junqi Kuang
human	WB	Cell Rep; FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression; Authors - Yanhua Zhang
human	WB,CoIP	Cancer Res; BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription.; Authors - Harte Mary T MT ; KD Validated
mouse	WB	Cell Mol Immunol; BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway.; Authors - Ran Zhao ; KO Validated