BRIP1(C-terminal) Polyclonal antibody

BRIP1(C-terminal) Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20983-1-AP

Synonyms

BACH1, BRIP1, BRIP1(C-terminal), FANCJ, OF



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20983-1-AP targets BRIP1(C-terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name BRCA1 interacting protein C-terminal helicase 1
Calculated molecular weight 141 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberNM_032043
Gene symbol BRIP1
Gene ID (NCBI) 83990
RRIDAB_2878782
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRIP1, also named as BACH1 and FANCJ, belongs to the DEAD box helicase family and DEAH subfamily. It is a DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. It is involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Defects in BRIP1 are a cause of susceptibility to breast cancer (BC). Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ). The antibody is specific to BRIP1.

Protocols

Product Specific Protocols
WB protocol for BRIP1(C-terminal) antibody 20983-1-APDownload protocol
IHC protocol for BRIP1(C-terminal) antibody 20983-1-APDownload protocol
IF protocol for BRIP1(C-terminal) antibody 20983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols