BVES Polyclonal antibody

BVES Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12920-1-AP

Synonyms

BVES, HBVES, POP1, POPDC1, Popeye protein 1

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 12920-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12920-1-AP (BVES antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12920-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12920-1-AP (BVES antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 12920-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12920-1-AP (BVES antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

12920-1-AP targets BVES in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BVES fusion protein Ag3593
• Full Name: blood vessel epicardial substance
• Calculated Molecular Weight: 360 aa, 41 kDa
• Observed Molecular Weight: 41-70 kDa
• GenBank Accession Number: BC034425
• Gene Symbol: BVES
• Gene ID (NCBI): 11149
• RRID: AB_10640584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BVES, also named as POP1, POPDC1 and hBVES, belongs to the popeye family. It is cell adhesion molecule involved in the establishment and/or maintenance of cell integrity. It is involved in the formation and regulation of the tight junction (TJ) paracellular permeability barrier in epithelial cells. It is also involved in striated muscle regeneration and repair and in the regulation of cell spreading. BVES is expressed in the progenitors of coronary artery smooth muscle cells as well as in differentiated smooth muscle cells.

Publications

Species	Application	Title
human	WB	Oncol Rep; MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer; Authors - Wang-Sheng Chen
human	IHC	Pathol Oncol Res; Abnormal expression of adhesion protein Bves is associated with gastric cancer progression and poor survival.; Authors - Luo Deng D

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