BCL2L1 Monoclonal antibody, PBS Only (Detector)

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  WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

  Jurkat, Ramos, K-562 and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66020-1-Ig (same clone as 66020-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Bcl-XL antibody (66020-1-Ig, Clone: 4C12A6 ) at dilution of 1:1355 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-XL antibody (66020-1-Ig, Clone: 4C12A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50613-1

  Cytometric bead array standard curve of MP50613-1, Bcl-xL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66020-2-PBS. Detection antibody: 66020-1-PBS. Standard:Ag18037. Range: 1.563-100 ng/mL.

BCL2L1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 66020-1-PBS

Clone No.4C12A6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Bcl-xL, 4C12A6, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1

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Product Information

66020-1-PBS targets BCL2L1 as part of a matched antibody pair:

MP50613-1: 66020-2-PBS capture and 66020-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18037

  Product name: Recombinant human Bcl-xL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-233 aa of BC019307

  Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK

• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: BCL2L1
• Gene ID (NCBI): 598
• RRID: AB_11042315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07817
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.