Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4C12A6

Cat no : 66020-1-Ig

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL



Tested Applications

Positive WB detected inJurkat cells, A431 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue, human testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66020-1-Ig is Bcl-XL Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag18037
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IHC protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IF protocol for Bcl-XL antibody 66020-1-IgDownload protocol
FC protocol for Bcl-XL antibody 66020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cancer Res Clin Oncol

Pathobiological role of cleft palate transmembrane protein 1 family proteins in oral squamous cell carcinoma.

Authors - Keisuke Inoue
humanWB

Biochim Biophys Acta Mol Basis Dis

Downregulation of TIMP2 attenuates sepsis-induced AKI through the NF-κb pathway.

Authors - Yi-Ming Li
humanWB

Toxicol Lett

Insights into mechanisms of pranoprofen-induced apoptosis and necroptosis in human corneal stromal cells.

Authors - Yani Lin
humanWB

Mol Cancer Res

A novel regulatory mechanism of Pim-3 kinase stability and its involvement in pancreatic cancer progression.

Authors - Fei Zhang
humanWB

Biochem Pharmacol

Targeting PI3K, mTOR, ERK, and Bcl-2 signaling network shows superior antileukemic activity against AML ex vivo.

Authors - Yongwei Su
humanWB

Antioxid Redox Signal

MCUR1-Mediated Mitochondrial Calcium Signaling Facilitates Cell Survival of Hepatocellular Carcinoma via Reactive Oxygen Species-Dependent P53 Degradation.

Authors - Tingting Ren