|Positive WB detected in||Jurkat cells, A431 cells, HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human lymphoma tissue, human testis tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:600|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66020-1-Ig is Bcl-XL Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Bcl-XL fusion protein Ag18037|
|Full Name||BCL2-like 1|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC019307|
|Gene ID (NCBI)||598|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.
|Product Specific Protocols|
|WB protocol for Bcl-XL antibody 66020-1-Ig||Download protocol|
|IHC protocol for Bcl-XL antibody 66020-1-Ig||Download protocol|
|IF protocol for Bcl-XL antibody 66020-1-Ig||Download protocol|
|FC protocol for Bcl-XL antibody 66020-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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