Biotin Recombinant monoclonal antibody

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  WB analysis using 86349-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot using biotin-66031 (alpha Tubulin antibody) at a dilution of 1:10000 followed by 86349-3-RR (Biotin antibody) at a dilution of 1:10000 and HRP-conjugated Goat Anti-Rabbit lgG(H+L), incubated at 37℃ 1h.

Biotin Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86349-3-RR

Clone No.242355E7

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, ELISA

Synonyms

Coenzyme R, Vitamin B7, Vitamin H

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86349-3-RR targets Biotin in WB, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Biotin
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Biotin, also called vitamin H or vitamin B7, is a water-soluble vitamin required by all organisms (PMID: 12055344). It serves as a necessary cofactor for enzymes involved in carboxylation reactions essential for gluconeogenesis, lipid metabolism, and amino acid catabolism (PMID: 38825069). Biotin is often used to label proteins and nucleic acids. This anti-biotin antibody can be used for detecting biotinylated molecules.