APC15 Polyclonal antibody

APC15 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20409-1-AP

Synonyms

APC15, C11orf51, HSPC020

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Validation Data Gallery

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  WB analysis of HT-29 using 20409-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20409-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20409-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HT-29 cells, HEK-293 cells
• Positive IHC detected in: human lung tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20409-1-AP is APC15 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APC15 fusion protein Ag14237
• Full Name: chromosome 11 open reading frame 51
• Calculated Molecular Weight: 121 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC005156
• Gene Symbol: C11orf51
• Gene ID (NCBI): 25906
• RRID: AB_10694279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.