VIPAS39 Polyclonal antibody

VIPAS39 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20771-1-AP

Synonyms

C14orf133, hSPE 39, Protein spe 39 homolog, VIPAR, VIPAS39, VPS33B interacting protein

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Validation Data Gallery

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  WB analysis using 20771-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 20771-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20771-1-AP (VIPAS39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 20771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20771-1-AP (VIPAS39 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20771-1-AP

  Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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  IF Staining of HEK-293 using 20771-1-AP

  Immunofluorescent analysis of 293 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 20771-1-AP

  Immunofluorescent analysis of HepG2 cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 20771-1-AP

  Immunofluorescent analysis of Hela cells, using C14orf133 antibody 20771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, human kidney tissue, HepG2 cells, L02 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells, HEK-293 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

20771-1-AP targets VIPAS39 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VIPAS39 fusion protein Ag14762
• Full Name: chromosome 14 open reading frame 133
• Calculated molecular weight: 493 aa, 57 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC015054
• Gene symbol: C14orf133
• Gene ID (NCBI): 63894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; FERARI is required for Rab11-dependent endocytic recycling.; Authors - Jachen A Solinger
human	WB	Virus Genes; Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7.; Authors - Ye Li

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