TICRR Polyclonal antibody

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  WB analysis using 30664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30664-1-AP (C15orf42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30664-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C15orf42 antibody (30664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

C15orf42 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30664-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

C15orf42, FLJ41618, TICRR, Treslin

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30664-1-AP targets C15orf42 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33632

  Product name: Recombinant human C15orf42 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 241-590 aa of BC033209

  Sequence: VELEMQASGLPKLRIKKIDPSSSLEAEPLSKEESSLGEESFLPALSMPRASRSLSKPEPTYVSPPCPRLSHSTPGKSRGQTYICQACTPTHGPSSTPSPFQTDGVPWTPSPKHSGKTTPDIIKDWPRRKRAVGCGAGSSSGRGEVGADLPGSLSLLESEGKDHGLELSIHRTPILEDFELEGVCQLPDQSPPRNSMPKAEEASSWGQFGLSSRKRVLLAKEEADRGAKRICDLREDSEVSKSKEGSPSWSAWQLPSTGDEEVFVSGSTPPPSCAVRSCLSASALQALTQSPLLFQGKTPSSQSKDPRDEDVDVLPSTVEDSPFSRAFSRRRPISRTYTRKKLMGTWLEDL

• Full Name: chromosome 15 open reading frame 42
• Observed Molecular Weight: 210-250 kDa
• GenBank Accession Number: BC033209
• Gene Symbol: C15orf42
• Gene ID (NCBI): 90381
• RRID: AB_3669748
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z2Z1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C15orf42 (TOPBP1 interacting checkpoint and replication regulator), also known as Treslin. It is expected to be located in cytosol and nucleoplasm, the protein is mainly expressed in bone marrow, esophagus, and lymphoid tissue. The protein C15orf42 enables chromatin binding activity and it is involved in regulation of DNA-dependent DNA replication initiation. And the calculated molecular weight of C15orf42 is 210 kDa, the 250 kDa band has been reported in the literature (PMID: 30037903).