C16orf70 Polyclonal antibody

C16orf70 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20602-1-AP

Synonyms

C16orf6, C16orf70, lin 10, LIN10, UPF0183 protein C16orf70

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Validation Data Gallery

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  WB analysis using 20602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20602-1-AP (C16orf70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 20602-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20602-1-AP (C16orf70 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 20602-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20602-1-AP (C16orf70 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20602-1-AP is C16orf70 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C16orf70 fusion protein Ag14576
• Full Name: chromosome 16 open reading frame 70
• Calculated Molecular Weight: 422 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC004556
• Gene Symbol: C16orf70
• Gene ID (NCBI): 80262
• RRID: AB_10694825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C20orf30 has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 48 kDa, and our antibody specially recognises the 48 kDa protein.