COA6 Polyclonal antibody

• Featured Product
• KD/KO Validated

COA6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24209-1-AP

Synonyms

C1orf31, COA6, Cytochrome c oxidase assembly factor 6 homolog

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Validation Data Gallery

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  WB analysis of HEK-293 using 24209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24209-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24209-1-AP (COA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24209-1-AP

  IP result of anti-COA6 (IP:24209-1-AP, 4ug; Detection:24209-1-AP 1:500) with HEK-293 cells lysate 1760ug.

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  IHC staining of human kidney using 24209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24209-1-AP (COA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24209-1-AP (COA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 24209-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf31 antibody (24209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

24209-1-AP targets COA6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COA6 fusion protein Ag21525
• Full Name: chromosome 1 open reading frame 31
• Calculated Molecular Weight: 125 aa, 14 kDa
• Observed Molecular Weight: 10 - 18 kDa
• GenBank Accession Number: BC116455
• Gene Symbol: COA6
• Gene ID (NCBI): 388753
• RRID: AB_2879459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1orf31, also known as COA6, is a conserved mitochondrial protein required for respiratory complex IV biogenesis. Recently study reported that C1orf31 is required for maintenance of cytochrome c oxidase (CcO) subunit levels, and has role in the Cu delivery pathway to CcO. Defects in C1orf31 is associated with mitochondrial respiratory chain disease (MRCD). (24549041)

Publications

Species	Application	Title
human	WB	Hum Mol Genet; Copper supplementation restores cytochrome c oxidase assembly defect in a mitochondrial disease model of COA6 deficiency.; Authors - Alok Ghosh ; KD Validated
human	WB	Cell Rep; COA6 Is Structurally Tuned to Function as a Thiol-Disulfide Oxidoreductase in Copper Delivery to Mitochondrial Cytochrome c Oxidase.; Authors - Shivatheja Soma
human	WB,IF	Int J Mol Sci; High Expression of COA6 Is Related to Unfavorable Prognosis and Enhanced Oxidative Phosphorylation in Lung Adenocarcinoma; Authors - Ming Zhang ; KD Validated
human	IHC	Heliyon; Prognostic value and immunological function of cuproptosis-related genes in lung adenocarcinoma; Authors - Liming Zhang