C1orf58 Polyclonal antibody, PBS Only

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  WB analysis using 26109-1-AP (same clone as 26109-1-PBS)

  HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 26109-1-AP (same clone as 26109-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 26109-1-AP (same clone as 26109-1-PBS)

  IP result of anti-C1orf58 (IP:26109-1-AP, 4ug; Detection:26109-1-AP 1:300) with HepG2 cells lysate 1680 ug. This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 26109-1-AP (same clone as 26109-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 26109-1-AP (same clone as 26109-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 26109-1-AP (same clone as 26109-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf58 antibody (26109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

C1orf58 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 26109-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

BROX, BROFTI, BRO1 domain-containing protein BROX, BRO1 domain containing protein BROX, BRO1 domain- and CAAX motif-containing protein

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Product Information

26109-1-PBS targets C1orf58 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23443

  Product name: Recombinant human C1orf58 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-355 aa of BC113635

  Sequence: MTHWFHRNPLKATAPVSFNYYGVVTGPSASKICNDLRSSRARLLELFTDLSCNPEMMKNAADSYFSLLQGFINSLDESTQESKLRYIQNFKWTDTLQGQVPSAQQDAVFELISMGFNVALWYTKYASRLAGKENITEDEAKEVHRSLKIAAGIFKHLKESHLPKLITPAEKGRDLESRLIEAYVIQCQAEAQEVTIARAIELKHAPGLIAALAYETANFYQKADHTLSSLEPAYSAKWRKYLHLKMCFYTAYAYCYHGETLLASDKCGEAIRSLQEAEKLYAKAEALCKEYGETKGPGPTVKPSGHLFFRKLGNLVKNTLEKCQRENGFIYFQKIPTEAPQLELKANYGLVEPIP

• Full Name: chromosome 1 open reading frame 58
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC113635
• Gene Symbol: C1orf58
• Gene ID (NCBI): 148362
• RRID: AB_2880382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VW32
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.