C1orf58 Polyclonal antibody

C1orf58 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26109-1-AP

Synonyms

BROFTI, Brox, C1orf58, RP11 452F19.1

---

Validation Data Gallery

• 

  WB analysis using 26109-1-AP

  HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26109-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 26109-1-AP

  IP result of anti-C1orf58 (IP:26109-1-AP, 4ug; Detection:26109-1-AP 1:300) with HepG2 cells lysate 1680 ug.

• 

  IHC staining of human breast cancer using 26109-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26109-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 26109-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 26109-1-AP (C1orf58 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26109-1-AP is C1orf58 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf58 fusion protein Ag23443
• Full Name: chromosome 1 open reading frame 58
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC113635
• Gene Symbol: C1orf58
• Gene ID (NCBI): 148362
• RRID: AB_2880382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.