C22orf32 Polyclonal antibody

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  WB analysis using 29313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29313-1-AP (C22orf32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 29313-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C22orf32 antibody (29313-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

C22orf32 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29313-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SMDT1, EMRE, DDDD

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Tested Applications

• Positive WB detected in: SW480 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29313-1-AP targets C22orf32 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29162

  Product name: Recombinant human C22orf32 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-107 aa of BC024237

  Sequence: MASGAARWLVLAPVRSGALRSGPSLRKDGDVSAAWSGSGRSLVPSGSVIVTRSGAILPKPVKMSFGLLRVFSIVIPFLYVGTLISKNFAALLEEHDIFVPEDDDDDD

• Full Name: chromosome 22 open reading frame 32
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC024237
• Gene Symbol: C22orf32
• Gene ID (NCBI): 91689
• RRID: AB_3669681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4I9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C22orf32, also known as SMDT1 or EMRE, is a 10 kDa single-channel transmembrane that contains a mitochondrial targeting sequence, a transmembrane region, and a conserved aspart-rich C-terminal. It acts as a subunit of the MCU complex and increases mCa2+ uptake by activating the MCU function (PMID: 38417574).