C22orf41 Polyclonal antibody

C22orf41 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24867-1-AP

Synonyms

C22orf41, SYCE3, THEG 2, THEG2

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Validation Data Gallery

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  IHC staining of mouse testis using 24867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24867-1-AP (C22orf41 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24867-1-AP (C22orf41 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 24867-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24867-1-AP (C22orf41 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 24867-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24867-1-AP (C22orf41 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 24867-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24867-1-AP (C22orf41 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 24867-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24867-1-AP (C22orf41 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24867-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24867-1-AP (C22orf41 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IHC detected in: mouse testis tissue, rat testis tissue, human small intestine tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

24867-1-AP targets C22orf41 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C22orf41 fusion protein Ag21756
• Full Name: chromosome 22 open reading frame 41
• Calculated Molecular Weight: 88 aa, 11 kDa
• GenBank Accession Number: BC127859
• Gene Symbol: C22orf41
• Gene ID (NCBI): 644186
• RRID: AB_2879765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C22orf41 is also named as SYCE3 and THEG2. C22orf41 is major component of the transverse central element of synaptonemal complexes (SCS). It formed between homologous chromosomes during meiotic prophase. SYCE3 is a small protein of 88 amino acids that is essential for SC assembly, meiotic division, and fertility (PMID:21637789).

Publications

Species	Application	Title
mouse	WB,IF	J Steroid Biochem Mol Biol; Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.; Authors - Qi Wang
mouse	WB,IF	Cell Rep; Male fertility in mice requires classical and nonclassical androgen signaling.; Authors - Paul S Cooke