C3/C3b/C3c Monoclonal antibody

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  WB analysis using 66157-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 66157-1-Ig

  human plasma was subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 66157-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66157-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66157-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66157-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

C3/C3b/C3c Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66157-1-Ig

Clone No.2A6B3

Publications(4)

Reviews (2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

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Tested Applications

• Positive WB detected in: HUVEC cells, human blood, human plasma, HepG2 cells, J774A.1 cells, RAW 264.7 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Product Information

66157-1-Ig targets C3/C3b/C3c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3/C3b/C3c fusion protein Ag15955
• Full Name: complement component 3
• Calculated Molecular Weight: 1663 aa, 187 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC150179
• Gene Symbol: C3
• Gene ID (NCBI): 718
• RRID: AB_2881553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01024
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IF	J Neuroinflammation; Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.; Authors - Qing Liu
mouse	WB,ELISA	Virulence; HylS', a fragment of truncated hyaluronidase of Streptococcus suis, contributes to immune evasion by interaction with host complement factor C3b; Authors - Jiajia Xu
mouse	IF	Int J Oral Sci; Fibroblast derived C3 promotes the progression of experimental periodontitis through macrophage M1 polarization and osteoclast differentiation; Authors - Feilong Ren
rat	WB	Neurochem Int; Quantitative iTRAQ-based proteomic analysis of piperine protected cerebral ischemia/reperfusion injury in rat brain.; Authors - Yichun Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (08-01-2024)

LPS induction model Fixative: Paraformaldehyde 4% Permeabilization: 10 min; RT; 0.2% Triton X-100 Blocking: 2 h; RT; 10% Normal donkey serum; 1% BSA Primary antibody: 1 night; 4°C Secondary antibody: ab150112 (Abcam); 1h30; RT Antibodies diluted in blocking solution

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Rat Kidney

C3/C3b/C3c Antibody Immunofluorescence validation (1/200 dilution) in Rat Kidney (Cat no:66157-1-Ig)

FH

Neil (Verified Customer) (05-10-2019)

The Western Blot came out spectacularly! I saw the bands I expected to see, and everything was clear and distinct. I would recommend this to anyone looking to identify C3.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Synovial Fluid