C3/C3b/C3c Monoclonal antibody

C3/C3b/C3c Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2A6B3

Cat no : 66157-1-Ig

Synonyms

ARMD9, ASP, C3, C3b, C3c, Complement C3, complement component 3, CPAMD1



Tested Applications

Positive WB detected inhuman plasma tissue, human blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66157-1-Ig targets C3/C3b/C3c in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C3/C3b/C3c fusion protein Ag15955
Full Name complement component 3
Calculated molecular weight 1663 aa, 187 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC150179
Gene symbol C3
Gene ID (NCBI) 718
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

Protocols

Product Specific Protocols
WB protocol for C3/C3b/C3c antibody 66157-1-IgDownload protocol
IHC protocol for C3/C3b/C3c antibody 66157-1-IgDownload protocol
IF protocol for C3/C3b/C3c antibody 66157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neurochem Int

Quantitative iTRAQ-based proteomic analysis of piperine protected cerebral ischemia/reperfusion injury in rat brain.

Authors - Yichun Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Neil (Verified Customer) (05-10-2019)

The Western Blot came out spectacularly! I saw the bands I expected to see, and everything was clear and distinct. I would recommend this to anyone looking to identify C3.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: Synovial Fluid