C3/C3b/C3c Monoclonal antibody
C3/C3b/C3c Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
human and More (1)
WB, IHC, IF, ELISA
Cat no : 66157-1-Ig
|Positive WB detected in||human plasma tissue, human blood tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66157-1-Ig targets C3/C3b/C3c in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||C3/C3b/C3c fusion protein Ag15955|
|Full Name||complement component 3|
|Calculated molecular weight||1663 aa, 187 kDa|
|Observed molecular weight||115 kDa|
|GenBank accession number||BC150179|
|Gene ID (NCBI)||718|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.
|Product Specific Protocols|
|WB protocol for C3/C3b/C3c antibody 66157-1-Ig||Download protocol|
|IHC protocol for C3/C3b/C3c antibody 66157-1-Ig||Download protocol|
|IF protocol for C3/C3b/C3c antibody 66157-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Neil (Verified Customer) (05-10-2019)
The Western Blot came out spectacularly! I saw the bands I expected to see, and everything was clear and distinct. I would recommend this to anyone looking to identify C3.