C3/C3b/C3c Monoclonal antibody
C3/C3b/C3c Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
2A6B3
Cat no : 66157-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue, human blood tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
66157-1-Ig targets C3/C3b/C3c in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | C3/C3b/C3c fusion protein Ag15955 |
| Full Name | complement component 3 |
| Calculated molecular weight | 1663 aa, 187 kDa |
| Observed molecular weight | 115 kDa |
| GenBank accession number | BC150179 |
| Gene symbol | C3 |
| Gene ID (NCBI) | 718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C3/C3b/C3c antibody 66157-1-Ig | Download protocol |
| IHC protocol for C3/C3b/C3c antibody 66157-1-Ig | Download protocol |
| IF protocol for C3/C3b/C3c antibody 66157-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurochem Int Quantitative iTRAQ-based proteomic analysis of piperine protected cerebral ischemia/reperfusion injury in rat brain. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Neil (Verified Customer) (05-10-2019) | The Western Blot came out spectacularly! I saw the bands I expected to see, and everything was clear and distinct. I would recommend this to anyone looking to identify C3.
|
