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C3/C3b/C3c Antibody 1 Publications

Mouse Monoclonal| Catalog number: 66157-1-Ig |CloneNo.: 2A6B3

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Con: 53 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

C3/C3b/C3c Antibody WB human plasma tissue 66157-1-Ig

human plasma tissue were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

C3/C3b/C3c Antibody IHC human liver tissue 66157-1-Ig

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens).

C3/C3b/C3c Antibody IHC human liver tissue 66157-1-Ig

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens).

C3/C3b/C3c Antibody IF HepG2 cells 66157-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human
Positive WB detected in:
human plasma tissue, human blood tissue
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
C3/C3b/C3c fusion protein Ag15955
Full name:
complement component 3
Calculated molecular weight:
1663aa,187 kDa
Observed molecular weight:
115 kDa
GenBank accession number:
BC150179
Gene ID (NCBI):
718
Gene symbol
C3
Synonyms
ARMD9, ASP, C3, C3b, C3c, Complement C3, complement component 3, CPAMD1

Background

The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognize C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.


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