C4BPA Polyclonal antibody

C4BPA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11819-1-AP

Synonyms

C4BP, C4BPA, Proline rich protein, PRP



Tested Applications

Positive WB detected inhuman blood tissue, human plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11819-1-AP targets C4BPA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C4BPA fusion protein Ag2387
Full Name complement component 4 binding protein, alpha
Calculated molecular weight 597 aa, 67 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC022312
Gene symbol C4BPA
Gene ID (NCBI) 722
RRIDAB_2877796
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP alpha chain (C4BPA).

Protocols

Product Specific Protocols
WB protocol for C4BPA antibody 11819-1-APDownload protocol
IHC protocol for C4BPA antibody 11819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Brain

Extracellular vesicle biomarkers for complement dysfunction in schizophrenia

Authors - Ting Xue
humanIHC

Oncotarget

Hepatitis B virus X protein up-regulates C4b-binding protein α through activating transcription factor Sp1 in protection of hepatoma cells from complement attack.

Authors - Guoxing Feng
humanWB

BMC Cancer

The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.

Authors - Ping Zhao
humanWB

World J Surg Oncol

Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma

Authors - Wei Dong