C4BPB Polyclonal antibody

C4BPB Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 15837-1-AP


C4b binding protein beta chain, C4BP, C4BPB

Tested Applications

Positive WB detected inhuman plasma tissue, HeLa cells, mouse ovary tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15837-1-AP targets C4BPB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C4BPB fusion protein Ag8587
Full Name complement component 4 binding protein, beta
Calculated Molecular Weight 251 aa, 28 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC005378
Gene Symbol C4BPB
Gene ID (NCBI) 725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP beta chain (C4BPB).


Product Specific Protocols
WB protocol for C4BPB antibody 15837-1-APDownload protocol
IHC protocol for C4BPB antibody 15837-1-APDownload protocol
IF protocol for C4BPB antibody 15837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



BMC Cancer

The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.

Authors - Ping Zhao

World J Surg Oncol

Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma

Authors - Wei Dong