C7orf50 Monoclonal antibody

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  WB analysis using 68494-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68494-1-Ig (C7orf50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 68494-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68494-1-Ig (C7orf50 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 68494-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 68494-1-Ig (C7orf50 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68494-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C7orf50 antibody (68494-1-Ig, Clone: 2A3G5 ) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

C7orf50 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68494-1-Ig

Clone No.2A3G5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2A3G5, FP15621, YCR016W

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Tested Applications

• Positive WB detected in: A431 cells, SW480 cells, HT-29 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, Ramos cells, THP-1 cells
• Positive IHC detected in: human liver tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68494-1-Ig targets C7orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15142

  Product name: Recombinant human C7orf50 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-194 aa of BC006224

  Sequence: MAKQKRKVPEVTEKKNKKLKKASAEGPLLGPEAAPSGEGAGSKGEAVLRPGLDAEPELSPEEQRVLERKLKKERKKEERQRLREAGLVAQHPPARRSGAELALDYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQLLS

• Full Name: chromosome 7 open reading frame 50
• Calculated Molecular Weight: 194 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006224
• Gene Symbol: C7orf50
• Gene ID (NCBI): 84310
• RRID: AB_3085205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BRJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7orf50 (Chromosome 7, Open Reading Frame 50) is ubiquitously expressed in the kidneys, brain, fat, prostate, spleen, among 22 other tissues and demonstrates low tissue specificity.