C7orf50 Monoclonal antibody, PBS Only

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  WB analysis using 68494-1-Ig (same clone as 68494-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68494-1-Ig (C7orf50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68494-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68494-1-Ig (same clone as 68494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68494-1-Ig (C7orf50 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68494-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 68494-1-Ig (same clone as 68494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 68494-1-Ig (C7orf50 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68494-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68494-1-Ig (same clone as 68494-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C7orf50 antibody (68494-1-Ig, Clone: 2A3G5 ) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68494-1-PBS in a different storage buffer formulation.

C7orf50 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68494-1-PBS

Clone No.2A3G5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2A3G5, FP15621, YCR016W

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Product Information

68494-1-PBS targets C7orf50 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15142

  Product name: Recombinant human C7orf50 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-194 aa of BC006224

  Sequence: MAKQKRKVPEVTEKKNKKLKKASAEGPLLGPEAAPSGEGAGSKGEAVLRPGLDAEPELSPEEQRVLERKLKKERKKEERQRLREAGLVAQHPPARRSGAELALDYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQLLS

• Full Name: chromosome 7 open reading frame 50
• Calculated Molecular Weight: 194 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006224
• Gene Symbol: C7orf50
• Gene ID (NCBI): 84310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BRJ6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

C7orf50 (Chromosome 7, Open Reading Frame 50) is ubiquitously expressed in the kidneys, brain, fat, prostate, spleen, among 22 other tissues and demonstrates low tissue specificity.