C9orf78 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86612-1-RR (same clone as 86612-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86612-1-RR (C9orf78 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86612-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86612-1-RR (same clone as 86612-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf78 antibody (86612-1-RR, Clone: 251553H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86612-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02557-1

  The mean C9orf78 concentration was determined to be 447.5 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load.

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  Cytometric bead array standard curve of MP02557-1

  Cytometric bead array standard curve of MP02557-1, C9orf78 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86612-1-PBS. Detection antibody: 86612-2-PBS. Standard: Ag5919. Range: 3.125-200 ng/mL.

C9orf78 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 86612-1-PBS

Clone No.251553H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

CSU2, HCA59, Hepatocellular carcinoma-associated antigen 59, HSPC220, TLS1

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Product Information

86612-1-PBS targets C9orf78 as part of a matched antibody pair:

MP02557-1: 86612-1-PBS capture and 86612-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5919

  Product name: Recombinant human C9orf78 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-289 aa of BC007664

  Sequence: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY

• Full Name: chromosome 9 open reading frame 78
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC007664
• Gene Symbol: C9orf78
• Gene ID (NCBI): 51759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZ63
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.