Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, RAW 264.7 cells, Neuro-2a cells, NIH/3T3 cells, C2C12 cells, PC-12 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86612-1-RR targets C9orf78 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5919 Product name: Recombinant human C9orf78 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-289 aa of BC007664 Sequence: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY Predict reactive species |
| Full Name | chromosome 9 open reading frame 78 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC007664 |
| Gene Symbol | C9orf78 |
| Gene ID (NCBI) | 51759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZ63 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.
