CA8 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86004-2-RR (same clone as 86004-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86004-2-RR (CA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86004-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86004-2-RR (same clone as 86004-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86004-2-RR (CA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86004-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86004-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86004-2-RR against Human CA8 were performed. The affinity constant is below 1 pM.

CA8 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86004-2-PBS

Clone No.250429C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

carbonic anhydrase VIII, CA 8, CA VIII, CALS, Carbonic anhydrase-related protein

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Product Information

86004-2-PBS targets CA8 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3068

  Product name: Recombinant human CA8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-143 aa of BC015531

  Sequence: MADLSFIEDTVAFPEKEEDEEEEEEGVEWGYEEGVEWGLVFPDANGEYQSPINLNSREARYDPSLLDVRLSPNYVVCRDCEVTNDGHTIQVILKSKSVLSGGPLPQGHEFELYEVRFHWGRENQRGSEHTVNFKAFPMEVRIT

• Full Name: carbonic anhydrase VIII
• Calculated Molecular Weight: 290 aa, 32 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC015531
• Gene Symbol: CA8
• Gene ID (NCBI): 767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35219
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).