CACNA1E Polyclonal antibody

• 

  WB analysis using 27225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27225-1-AP (CACNA1E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 27225-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 27225-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CACNA1E Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27225-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CACH6, CACNL1A6, Cav2.3

---

Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27225-1-AP targets CACNA1E in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACNA1E fusion protein Ag25944
• Full Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
• Calculated Molecular Weight: 262 kDa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: NM_000721
• Gene Symbol: CACNA1E
• Gene ID (NCBI): 777
• RRID: AB_2880808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15878
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACNA1E is a gene encoding the ion-conducting α1 subunit of R-type voltage-dependent calcium channels, genetic variability of CACNA1E is associated to risk of type 2 diabetes, insulin resistance and impaired insulin secretion in nondiabetic subjects.

Publications

Species	Application	Title
mouse	WB	Elife; β2-subunit alternative splicing stabilizes Cav2.3 Ca2+ channel activity during continuous midbrain dopamine neuron-like activity.; Authors - Anita Siller