Tested Applications
Positive WB detected in | rat brain tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
27225-1-AP targets CACNA1E in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CACNA1E fusion protein Ag25944 Predict reactive species |
Full Name | calcium channel, voltage-dependent, R type, alpha 1E subunit |
Calculated Molecular Weight | 262 kDa |
Observed Molecular Weight | 240 kDa |
GenBank Accession Number | NM_000721 |
Gene Symbol | CACNA1E |
Gene ID (NCBI) | 777 |
RRID | AB_2880808 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q15878 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CACNA1E is a gene encoding the ion-conducting α1 subunit of R-type voltage-dependent calcium channels, genetic variability of CACNA1E is associated to risk of type 2 diabetes, insulin resistance and impaired insulin secretion in nondiabetic subjects.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CACNA1E antibody 27225-1-AP | Download protocol |
IHC protocol for CACNA1E antibody 27225-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |