CACNG3 Polyclonal antibody

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  WB analysis of HEK-293 using 13729-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13729-1-AP (CACNG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13729-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13729-1-AP (CACNG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13729-1-AP (CACNG3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CACNG3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13729-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Voltage-dependent calcium channel gamma-3 subunit, Transmembrane AMPAR regulatory protein gamma-3, TARP gamma-3, Neuronal voltage-gated calcium channel gamma-3 subunit

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13729-1-AP targets CACNG3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACNG3 fusion protein Ag4730
• Full Name: calcium channel, voltage-dependent, gamma subunit 3
• Calculated Molecular Weight: 315 aa, 36 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC040005
• Gene Symbol: CACNG3
• Gene ID (NCBI): 10368
• RRID: AB_2070085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60359
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Voltage-dependent calcium channels couple membrane depolarization in a number of cellular processes. These activities are regulated by distinct channels composed of alpha-1, beta, alpha-2/delta, and gamma subunits. CACNG3, one of the eight gamma subunits in human, is a type I transmembrane AMPA receptor regulatory protein (TARP). TARPs regulate both trafficking and channel gating of the AMPA receptors.

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer; Integrated profiling identifies CACNG3 as a prognostic biomarker for patients with glioma; Authors - Enfang Shan