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CACYBP Polyclonal antibody

CACYBP Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11745-1-AP

Synonyms

CACYBP, calcyclin binding protein, GIG5, hCacyBP, PNAS 107, RP1 102G20.6, S100A6 binding protein, S100A6BP, Siah interacting protein, SIP



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11745-1-AP targets CACYBP in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACYBP fusion protein Ag2340
Full Name calcyclin binding protein
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC022352
Gene symbol CACYBP
Gene ID (NCBI) 27101
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exsit as a dimer(PMID:17916393).

Protocols

Product Specific Protocols
WB protocol for CACYBP antibody 11745-1-APDownload protocol
IHC protocol for CACYBP antibody 11745-1-APDownload protocol
IF protocol for CACYBP antibody 11745-1-APDownload protocol
IP protocol for CACYBP antibody 11745-1-APDownload protocol
FC protocol for CACYBP antibody 11745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation.

Authors - Yi-Fan Lian
  • KD Validated