CACYBP Polyclonal antibody

• Featured Product
• KD/KO Validated

CACYBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11745-1-AP

Synonyms

Calcyclin, S100 alpha 6, PNAS 107, hCacyBP, GIG5

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Validation Data Gallery

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  WB analysis of HeLa using 11745-1-AP

  HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, and rat liver tissue were subjected to SDS PAGE followed by western blot with 11745-1-AP (CACYBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11745-1-AP

  IP result of anti-CACYBP (IP:11745-1-AP, 4ug; Detection:11745-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11745-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11745-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11745-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CACYBP antibody (11745-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11745-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CACYBP Polyclonal antibody (11745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

11745-1-AP targets CACYBP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACYBP fusion protein Ag2340
• Full Name: calcyclin binding protein
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC022352
• Gene Symbol: CACYBP
• Gene ID (NCBI): 27101
• RRID: AB_2070266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).

Publications

Species	Application	Title
human	WB,IHC,IF	J Exp Clin Cancer Res; Blockage of CacyBP inhibits macrophage recruitment and improves anti-PD-1 therapy in hepatocellular carcinoma; Authors - Jialiang Wang ; KD Validated
human	WB	Theranostics; CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation.; Authors - Yi-Fan Lian ; KD Validated
mouse	WB	Br J Pharmacol; A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration; Authors - Si-Yuan Yao
human	IF,WB	Cancer Res; MAB21L4 Deficiency Drives Squamous Cell Carcinoma via Activation of RET; Authors - Ankit Srivastava
human	WB	Am J Transl Res; Pan-analysis reveals CACYBP to be a novel prognostic and predictive marker for multiple cancers; Authors - Baosen Mo
human	WB	Carcinogenesis; CacyBP promotes the development of lung adenocarcinoma by regulating OTUD5; Authors - Mixue Bai