CAD Polyclonal antibody, PBS Only

• 

  WB analysis of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16617-1-AP (CAD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

  IP result of anti-CAD (IP:16617-1-AP, 4ug; Detection:16617-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 16617-1-AP (same clone as 16617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16617-1-AP (same clone as 16617-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 16617-1-AP (same clone as 16617-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 16617-1-AP (CAD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16617-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CAD Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16617-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Ammonium-dependent carbamoyl phosphate synthase, Aspartate carbamoyltransferase, Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase, CPS, CPSase

---

Product Information

16617-1-PBS targets CAD in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9912

  Product name: Recombinant human CAD protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 502-851 aa of BC014178

  Sequence: VAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

• Full Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• Calculated Molecular Weight: 2225 aa, 243 kDa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: BC014178
• Gene Symbol: CAD
• Gene ID (NCBI): 790
• RRID: AB_2878289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27708
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).