CAD Polyclonal antibody

CAD Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16617-1-AP

Synonyms

CAD



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16617-1-AP targets CAD in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAD fusion protein Ag9912
Full Name carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Calculated molecular weight 2225 aa, 243 kDa
Observed molecular weight 240 kDa
GenBank accession numberBC014178
Gene symbol CAD
Gene ID (NCBI) 790
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAD antibody 16617-1-APDownload protocol
IHC protocol for CAD antibody 16617-1-APDownload protocol
IF protocol for CAD antibody 16617-1-APDownload protocol
IP protocol for CAD antibody 16617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Carcinogenesis

Synergistic Targeting of CHK1 and mTOR in MYC-driven tumors.

Authors - Xiaoxue Song
  • KD Validated
humanWB

Theranostics

Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.

Authors - Gengyi Zou