Calretinin Polyclonal antibody

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• Calretinin Polyclonal antibody (12278-1-AP)

Calretinin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12278-1-AP Copied!

Publications (24)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CALB2, 29 kDa calbindin, CAB29, CAL2, calbindin 2

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  WB analysis using 12278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of fetal human brain using 12278-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 12278-1-AP

  IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cerebellum using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cerebellum using 12278-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MKN-45 using 12278-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MKN-45 cells using Calretinin antibody (12278-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human appendicitis tissue, human brain tissue, human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MKN-45 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12278-1-AP targets Calretinin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2924

  Product name: Recombinant human Calretinin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-271 aa of BC015484

  Sequence: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM

• Full Name: calbindin 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC015484
• Gene Symbol: Calretinin
• Gene ID (NCBI): 794
• RRID: AB_2228338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22676
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Publications

What published studies show

Calretinin Polyclonal antibody (Cat# 12278-1-AP) has 24 citations across journals including Science Advances, Molecular Psychiatry, Cell Reports, Journal of Translational Medicine, NeuroImage, iScience, Investigative Ophthalmology & Visual Science, and Frontiers in Molecular Biosciences. Research fields span neuroscience (cortical mapping, retinal development, GABAergic neurons), oncology (colorectal, renal, gastric cancer, mesothelioma), vascular biology (atherosclerosis), developmental biology, and peritoneal fibrosis.

Automatically generated from the 24 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Adv Sci (Weinh); Targeting DNA-LNPs to Endothelial Cells Improves Expression Magnitude, Duration, and Specificity.; Authors - Nicolas Marzolini
human	IF	Sci Adv; A cellular resolution atlas of Broca's area; Authors - Irene Costantini
mouse	IHC	Mol Psychiatry; Somatostatin-expressing neurons in the zona incerta regulate chronic stress response and modulate depression-like behaviors.; Authors - Hongling Guo
mouse	IF	J Transl Med; Plasma proteins and onset of atherosclerosis: integrating human plasma proteogenomics, transcriptomics and in vivo evidence.; Authors - Kuangyang Chen
human	IF	Cell Rep; Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment; Authors - Atsuko Yonemura
human	IF	J Med Chem; Immune Checkpoint-Modulating Photosensitizer That Targets BRD4 for Cancer Photoimmunotherapy; Authors - Peixia Li