Calretinin Polyclonal antibody

Calretinin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12278-1-AP


29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

Tested Applications

Positive WB detected infetal human brain tissue, human ileum tissue, human brain tissue, human colon tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman appendicitis tissue, mouse brain tissue, human brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12278-1-AP targets Calretinin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calretinin fusion protein Ag2924
Full Name calbindin 2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol CALB2
Gene ID (NCBI) 794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.


Product Specific Protocols
WB protocol for Calretinin antibody 12278-1-APDownload protocol
IHC protocol for Calretinin antibody 12278-1-APDownload protocol
IP protocol for Calretinin antibody 12278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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