Calmodulin Polyclonal antibody

Calmodulin Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10541-1-AP

Synonyms

CALM, CALM1, CALM2, CALM3, CALML2, Calmodulin, CaM, CAM1, CAM2, CAM3, CAMB, CAMC, CAMIII, PHKD, PHKD3



Tested Applications

Positive WB detected inRamos cells, mouse brain tissue, NCCIT cell, MCF-7 cells, HepG2 cells, human brain tissue, HeLa cells
Positive IP detected inRamos cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10541-1-AP targets Calmodulin in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calmodulin fusion protein Ag0827
Full Name calmodulin 3 (phosphorylase kinase, delta)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC006182
Gene symbol CALM3
Gene ID (NCBI) 808
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calmodulin (CaM) is a Ca(2+)-binding protein that transduces Ca2+-mediated signals by binding to and regulating the activity of hundreds of enzymes and non-enzymatic proteins. It is highly conserved across species and involved in many biological processes, including vesicle release, cell proliferation, and apoptosis. This antibody can recognize CALM1, CALM2 and CALM3.

Protocols

Product Specific Protocols
WB protocol for Calmodulin antibody 10541-1-APDownload protocol
IHC protocol for Calmodulin antibody 10541-1-APDownload protocol
IP protocol for Calmodulin antibody 10541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Oncotarget

PINK1 suppresses alpha-synuclein-induced neuronal injury: a novel mechanism in protein phosphatase 2A activation.

Authors - Weiwei Yang
mouseWB

Cell Rep

SLC22A14 is a mitochondrial riboflavin transporter required for sperm oxidative phosphorylation and male fertility.

Authors - Wenhua Kuang
humanWB

J Cardiovasc Transl Res

TRPC1 Deficiency Impairs the Endothelial Progenitor Cell Function via Inhibition of Calmodulin/eNOS Pathway.

Authors - Lai-Ling Du
humanWB

Biochem Biophys Res Commun

The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function.

Authors - Liang Zhou
humanIHC, WB

Cancer Cell Int

CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma.

Authors - Tao Liu