CARD11 Polyclonal antibody

• Featured Product
• KD/KO Validated

CARD11 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21741-1-AP

Synonyms

BIMP3, CARD11, Carma 1, CARMA1

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Validation Data Gallery

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  WB analysis using 21741-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21741-1-AP (CARD11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21741-1-AP

  WB result of CARD11 antibody (21741-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD11 transfected Raji cells.

• 

  WB analysis of mouse thymus using 21741-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21741-1-AP (CARD11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 21741-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21741-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 21741-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using CARD11 antibody (21741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, mouse thymus tissue
• Positive IHC detected in: human colon cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

The immunogen of 21741-1-AP is CARD11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CARD11 fusion protein Ag16468
• Full Name: caspase recruitment domain family, member 11
• Calculated Molecular Weight: 1154 aa, 133 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC111719
• Gene Symbol: CARD11
• Gene ID (NCBI): 84433
• RRID: AB_2878915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

Publications

Species	Application	Title
human	WB,IF	Front Immunol; Identification and Characterization of a Germline Mutation in CARD11 From a Chinese Case of B Cell Expansion With NF-κB and T Cell Anergy.; Authors - Peiwei Zhao
human	WB,IF	Front Immunol; A novel CARD11 germline mutation in a Chinese patient of B cell expansion with NF-κB and T cell anergy (BENTA) and literature review; Authors - Peiwei Zhao