|Positive WB detected in||Jurkat cells, HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66470-1-Ig targets Caspase 3(human specific) in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Caspase 3(human specific) fusion protein Ag25029|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Calculated molecular weight||277 aa, 32 kDa|
|Observed molecular weight||32-35 kDa, 19 kDa, 17 kDa|
|GenBank accession number||BC016926|
|Gene ID (NCBI)||836|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Casp3 (caspase 3), also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. This antibody is specific for human caspase 3, it can recognize the 32 kDa pro-caspase 3 as well as 17 and 19 kDa cleaved-caspase 3.
|Product Specific Protocols|
|WB protocol for Caspase 3(human specific) antibody 66470-1-Ig||Download protocol|
|IHC protocol for Caspase 3(human specific) antibody 66470-1-Ig||Download protocol|
|IF protocol for Caspase 3(human specific) antibody 66470-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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