Caspase 3(human specific) Monoclonal antibody
Caspase 3(human specific) Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2b
human and More (4)
WB, IHC, IF, ELISA
Cat no : 66470-1-Ig
|Positive WB detected in||Jurkat cells, HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 60 publications below|
|IHC||See 2 publications below|
|IF||See 4 publications below|
66470-1-Ig targets Caspase 3(human specific) in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, cow, zebrafish|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Caspase 3(human specific) fusion protein Ag25029|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Calculated molecular weight||277 aa, 32 kDa|
|Observed molecular weight||32-35 kDa, 19 kDa, 17 kDa|
|GenBank accession number||BC016926|
|Gene ID (NCBI)||836|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Casp3 (caspase 3), also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. This antibody is specific for human caspase 3, it can recognize the 32 kDa pro-caspase 3 as well as 17 and 19 kDa cleaved-caspase 3.
|Product Specific Protocols|
|WB protocol for Caspase 3(human specific) antibody 66470-1-Ig||Download protocol|
|IHC protocol for Caspase 3(human specific) antibody 66470-1-Ig||Download protocol|
|IF protocol for Caspase 3(human specific) antibody 66470-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis.
Restoration of L-OPA1 alleviates acute ischemic stroke injury in rats via inhibiting neuronal apoptosis and preserving mitochondrial function.
Chemical conjugation of FITC to track silica nanoparticles in vivo and in vitro: An emerging method to assess the reproductive toxicity of industrial nanomaterials.
UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer.
Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload.
Free Radic Biol Med
NAD+ depletion radiosensitizes 2-DG-treated glioma cells by abolishing metabolic adaptation.