CBR3 Polyclonal antibody, PBS Only

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  WB analysis using 15619-1-AP (same clone as 15619-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 15619-1-AP (same clone as 15619-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15619-1-AP (same clone as 15619-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15619-1-AP (CBR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

CBR3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15619-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

15619-1-PBS targets CBR3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8039

  Product name: Recombinant human CBR3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-277 aa of BC002812

  Sequence: MSSCSRVALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELLPIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW

• Full Name: carbonyl reductase 3
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC002812
• Gene Symbol: CBR3
• Gene ID (NCBI): 874
• RRID: AB_2070862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75828
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CBR3(Carbonyl reductase [NADPH] 3), which Belongs to the short-chain dehydrogenases/reductases (SDR) family, has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). The gene encodes a 31 kDa protein that can catalyze the reduction of many endogenous and xenobiotic carbonyl compounds, including steroids and prostaglandins. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein(PMID:9740676).