CFAP36 Polyclonal antibody, PBS Only

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  WB analysis of mouse lung using 24276-1-AP (same clone as 24276-1-PBS)

  mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24276-1-AP (same clone as 24276-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 24276-1-AP (same clone as 24276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 24276-1-AP (same clone as 24276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 24276-1-AP (same clone as 24276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lung using 24276-1-AP (same clone as 24276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24276-1-AP (same clone as 24276-1-PBS)

  Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24276-1-PBS in a different storage buffer formulation.

CFAP36 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24276-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CCDC104, Cilia- and flagella-associated protein 36, coiled coil domain containing 104, Coiled-coil domain-containing protein 104, UNQ163/PRO189

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Product Information

24276-1-PBS targets CFAP36 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17996

  Product name: Recombinant human CCDC104 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-342 aa of BC010011

  Sequence: MAAEEEDEVEWVVESIAGFLRGPDWSIPILDFVEQKCEVFDDEEESKLTYTEIHQEYKELVEKLLEGYLKEIGINEDQFQEACTSPLAKTHTSQAILQPVLAAEDFTIFKAMMVQKNIEMQLQAIRIIQERNGVLPDCLTDGSDVVSDLEHEEMKILREVLRKSKEEYDQEEERKRKKQLSEAKTEEPTVHSSEAAIMNNSQGDGEHFAHPPSEVKMHFANQSIEPLGRKVERSETSSLPQKGLKFPGLEHASIEGPIANLSVLGTEELRQREHYLKQKRDKLMSMRKDMRTKQIQNMEQKGKPTGEVEEMTEKPEMTAEEKQTLLKRRLLAEKLKEEVINK

• Full Name: coiled-coil domain containing 104
• Calculated Molecular Weight: 342 aa, 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC010011
• Gene Symbol: CCDC104
• Gene ID (NCBI): 112942
• RRID: AB_2879466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96G28
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CFAP36 (Cilia- and flagella-associated protein 36), also known as CCDC104(Coiled-coil domain-containing protein 104), whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.