CFAP36 Polyclonal antibody

• 

  WB analysis of mouse lung using 24276-1-AP

  mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24276-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24276-1-AP (CFAP36 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24276-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse stomach using 24276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 24276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 24276-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24276-1-AP (CCDC104 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 24276-1-AP

  Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

CFAP36 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24276-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCDC104, Cilia- and flagella-associated protein 36, coiled coil domain containing 104, Coiled-coil domain-containing protein 104, UNQ163/PRO189

---

Tested Applications

• Positive WB detected in: mouse lung tissue, HEK-293 cells
• Positive IHC detected in: mouse stomach tissue, human testis tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

24276-1-AP targets CFAP36 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17996

  Product name: Recombinant human CCDC104 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-342 aa of BC010011

  Sequence: MAAEEEDEVEWVVESIAGFLRGPDWSIPILDFVEQKCEVFDDEEESKLTYTEIHQEYKELVEKLLEGYLKEIGINEDQFQEACTSPLAKTHTSQAILQPVLAAEDFTIFKAMMVQKNIEMQLQAIRIIQERNGVLPDCLTDGSDVVSDLEHEEMKILREVLRKSKEEYDQEEERKRKKQLSEAKTEEPTVHSSEAAIMNNSQGDGEHFAHPPSEVKMHFANQSIEPLGRKVERSETSSLPQKGLKFPGLEHASIEGPIANLSVLGTEELRQREHYLKQKRDKLMSMRKDMRTKQIQNMEQKGKPTGEVEEMTEKPEMTAEEKQTLLKRRLLAEKLKEEVINK

• Full Name: coiled-coil domain containing 104
• Calculated Molecular Weight: 342 aa, 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC010011
• Gene Symbol: CCDC104
• Gene ID (NCBI): 112942
• RRID: AB_2879466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96G28
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CFAP36 (Cilia- and flagella-associated protein 36), also known as CCDC104(Coiled-coil domain-containing protein 104), whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cell; A conserved mechanism for the retrieval of polyubiquitinated proteins from cilia; Authors - Sven M Lange