CCDC104 Polyclonal antibody

CCDC104 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24276-1-AP

Synonyms

CCDC104

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Validation Data Gallery

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  WB analysis of mouse lung using 24276-1-AP

  mouse lung tissues were subjected to SDS PAGE followed by western blot with 24276-1-AP (CCDC104 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24276-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24276-1-AP (CCDC104 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24276-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 24276-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24276-1-AP (CCDC104 Antibody) at dilution of 1:50.

• 

  IF Staining of A431 using 24276-1-AP

  Immunofluorescent analysis of A431 cells using 24276-1-AP (CCDC104 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse lung tissue, HEK-293 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24276-1-AP is CCDC104 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC104 fusion protein Ag17996
• Full Name: coiled-coil domain containing 104
• Calculated Molecular Weight: 342 aa, 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC010011
• Gene Symbol: CCDC104
• Gene ID (NCBI): 112942
• RRID: AB_2879466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CCDC104(Coiled-coil domain-containing protein 104) protein, whose function is unknown, is expressed in various human tissues, including the brain, and is localized mainly to the nucleus, but is also found in the cytoplasm(PMID:19680650). It may act as an effector for ARL3. This protein has 2 isoforms produced by alternative splicing.