MCU/CCDC109A Polyclonal antibody

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• KD/KO Validated

MCU/CCDC109A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 26312-1-AP

Synonyms

C10orf42, Calcium uniporter protein, CCDC109A, MCU, MCU/CCDC109A

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Validation Data Gallery

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  WB analysis using 26312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26312-1-AP

  WB result of MCU/CCDC109A antibody (26312-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCU/CCDC109A transfected HeLa cells.

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  IHC staining of mouse kidney using 26312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26312-1-AP (MCU/CCDC109A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 26312-1-AP is MCU/CCDC109A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCU/CCDC109A fusion protein Ag24733
• Full Name: coiled-coil domain containing 109A
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC034235
• Gene Symbol: CCDC109A
• Gene ID (NCBI): 90550
• RRID: AB_2880474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCU, also known as CCDC109A, is highly conserved across all eukaryotes. The MCU protein is composed of two coiled-coil domains, two TMDs, and a short motif of amino acids between the two TMDs. Knockdown of MCU dramatically reduces mitochondrial Ca2+ uptake in isolated mitochondria, in permeabilized cells and living cells. MCU has 3 isoforms with MW 40,37 and 35 kDa (refer to UniProt). The observed MW of MCU is mainly between 30 to 35 kDa in paper (PMID: 26341627; 28337252).

Publications

Species	Application	Title
human	WB	Cell Death Differ; Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak.; Authors - Giovanni Quarato
human	WB	Environ Pollut; TDCPP and TiO2 NPs aggregates synergistically induce SH-SY5Y cell neurotoxicity by excessive mitochondrial fission and mitophagy inhibition; Authors - Ling Wang
human	WB	Cell Rep; A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson's disease; Authors - Vinita Bharat
mouse	WB	Int J Mol Sci; Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes; Authors - Tianyang Lan
mouse	WB	J Agric Food Chem; Puerarin Ameliorated PCOS through Preventing Mitochondrial Dysfunction Dependent on the Maintenance of Intracellular Calcium Homeostasis; Authors - Yu-Si Wang
mouse	WB	iScience; Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage; Authors - Tianyou Kan