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CCDC171 Polyclonal antibody

CCDC171 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28433-1-AP

Synonyms

bA536D16.1, bA778P13.1, C9orf93, CCDC171, DKFZp686P12113, FLJ39267, FLJ46740

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC171 Polyclonal antibody (28433-1-AP)

    WB analysis using 28433-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28433-1-AP (CCDC171 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28433-1-AP targets CCDC171 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC171 fusion protein Ag28926
Full Name chromosome 9 open reading frame 93
Calculated Molecular Weight 153 kDa
Observed Molecular Weight142 kDa
GenBank Accession NumberNM_173550
Gene Symbol C9orf93
Gene ID (NCBI) 203238
RRIDAB_2918164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCDC171 antibody 28433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Elisa (Verified Customer) (08-03-2022)

U2OS cells were lysates with RIPA buffer. 200ng for condition was loaded on the gel. . Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with CCDC171 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect CCDC171 signal (140-5kDa). b-tubulin (55kDa) was used as a loading control. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: U2OS

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS (Human Osteosarcoma) cells
CCDC171 Antibody Western Blot validation (1:1000 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:28433-1-AP)