Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28433-1-AP targets CCDC171 in WB, ELISA applications and shows reactivity with mouse, rat, human samples.
| Tested Reactivity | mouse, rat, human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28926 Product name: Recombinant human CCDC171 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-220 aa of NM_173550 Sequence: MNLNTSSNTGDTQRLKIASLDVKQILKNETELDITDNLRKKLHWAKKEKLEITTKHNAELASYESQIAKLRSEVEKGEALRQSLEYDLAVARKEAGLGRRAAEERLAEAHRIQEKLCAQNSELQAKTNETEKAFQTSQQKWKEECRRFEHDLEERDNMIQNCNREYDLLMKEKSRLEKTLQEALEKHQREKNEMESHIRETALEEFRLQEEQWEAERREL Predict reactive species |
| Full Name | chromosome 9 open reading frame 93 |
| Calculated Molecular Weight | 153 kDa |
| Observed Molecular Weight | 142 kDa |
| GenBank Accession Number | NM_173550 |
| Gene Symbol | CCDC171 |
| Gene ID (NCBI) | 203238 |
| RRID | AB_2918164 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6TFL3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC171 antibody 28433-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Elisa (Verified Customer) (08-03-2022) | U2OS cells were lysates with RIPA buffer. 200ng for condition was loaded on the gel. . Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with CCDC171 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect CCDC171 signal (140-5kDa). b-tubulin (55kDa) was used as a loading control. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: U2OS
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