Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue, rat stomach tissue, rat liver tissue, human stomach cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26013-1-AP targets CEP83 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22826 Product name: Recombinant human CCDC41 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 226-568 aa of BC125087 Sequence: MQATVRSLEAEKQSANLRAERLEKELQSSSEQNTFLINKLHKAEREINTLSSKVKELKHSNKLEITDIKLETARAKSELERERNKIQSELDGLQSDNEILKAAVEHHKVLLVEKDRELIRKVQAAKEEGYQKLVVLQDEKLELENRLADLEKMKVEHDVWRQSEKDQYEEKLRASQMAEEITRKELQSVRLKLQQQIVTIENAEKEKNENSDLKQQISSLQIQVTSLAQSENDLLNSNQMLKEMVERLKQECRNFRSQAEKAQLEAEKTLEEKQIQWLEEKHKLHERITDREEKYNQAKEKLQRAAIAQKKLEQDLELGCPSVTDTYRESVFPPPPLKRDLLK Predict reactive species |
| Full Name | coiled-coil domain containing 41 |
| Calculated Molecular Weight | 693 aa, 82 kDa |
| Observed Molecular Weight | 83 kDa, 68 kDa |
| GenBank Accession Number | BC125087 |
| Gene Symbol | CEP83 |
| Gene ID (NCBI) | 51134 |
| RRID | AB_2880334 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y592 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP83 (centrosomal protein, 83 kDa), also known as CCDC41 (coiled-coil domain containing 41), is required for ciliary vesicle docking to the mother centriole. It is a key component of the centriolar distal appendages. CCDC41 may collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly (PMID: 23530209). Alternative splicing results in transcript variants encoding two isoforms with calculated molecular weights of 83 kDa and 68 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP83 antibody 26013-1-AP | Download protocol |
| IHC protocol for CEP83 antibody 26013-1-AP | Download protocol |
| WB protocol for CEP83 antibody 26013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CEP83 Polyclonal antibody (Cat# 26013-1-AP) has 10 citations published in journals including Cell Research, Nature Communications, Developmental Cell, PLoS Biology, Cell Molecular Life Sciences, Journal of Cell Biology, Journal of Biological Chemistry, eLife, and bioRxiv. Research fields span ciliogenesis, centriolar distal appendage assembly, autophagy, cortical neurogenesis, centrosome proteomics, basal body organization, and spermiogenesis, with applications in WB and IF across human and mouse models.
| Species | Application | Title |
|---|---|---|
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
Dev Cell Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method | ||
PLoS Biol The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly | ||
Cell Mol Life Sci Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia. |
Reviews
What customers say
The product received two reviews with an average rating of 4.5/5. One reviewer used it for immunofluorescence on HEK293 cells at a 1:200 dilution, reporting clear staining for the mother centriole distal appendage marker with a 5-star rating. The other reviewer performed Western blot on U251 and Jurkat cell lysates at 1:1000 dilution, detecting the target protein on a 4-12% Bis-Tris gel with a 4-star rating. Both users reported successful results with standard protocols.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elisa (Verified Customer) (03-01-2022) | HEK293 cells stained for Hoechst (DNA marker, in green), Cep83 (mother centriole distal appendage marker, in magenta) and g-Tubulin (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 2' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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Sarah (Verified Customer) (07-03-2019) | Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-CCDC41 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.
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