CCDC47 Polyclonal antibody

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  WB analysis using 32080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32080-1-AP (CCDC47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 32080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 32080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 32080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC47 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32080-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Calumin, Coiled-coil domain-containing protein 47, GK001, MSTP041, PAT complex subunit CCDC47

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Tested Applications

• Positive WB detected in: Jurkat cells, L02 cells, RT-4 cells, mouse heart tissue, mouse lung tissue, rat heart tissue
• Positive IHC detected in: mouse cerebellum tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32080-1-AP targets CCDC47 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC47 fusion protein Ag36570
• Full Name: coiled-coil domain containing 47
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC008905
• Gene Symbol: CCDC47
• Gene ID (NCBI): 57003
• RRID: AB_3670185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96A33
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coiled-coil domain containing 47 (CCDC47, also known as THNS, GK001, and MSTP041) is an endoplasmic reticulum (ER) transmembrane protein involved in calcium signaling through utilization of its calcium binding-acidic luminal domain. It also interacts with the ERAD (endoplasmic reticulum-associated degradation) complex and is involved in ER stress relief (PMID: 28974366). The expression level of CCDC47 is associated with diabetic cardiomyopathy (PMID: 30140426). Its variant would cause trichohepatoneurodevelopmental syndrome (PMID: 38524542).