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  • KD/KO Validated

Cyclin D1 Monoclonal antibody

Cyclin D1 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat and More (4)







Cat no : 60186-1-Ig


B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31

Tested Applications

Positive WB detected inU2OS cells, A549 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells, SH-SY5Y cells, A431 cells, SW 1990 cells, HepG2 cells, HSC-T6 cells, PC-12 cells, HCT 116 cells, SK-N-SH cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:20000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60186-1-Ig targets Cyclin D1 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, goat, zebrafish, hamster, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cyclin D1 fusion protein Ag0689
Full Name cyclin D1
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000076
Gene Symbol CCND1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.


Product Specific Protocols
WB protocol for Cyclin D1 antibody 60186-1-IgDownload protocol
FC protocol for Cyclin D1 antibody 60186-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Janardhan (Verified Customer) (08-22-2020)

Antibody works ok, needs longer exposure

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Medulloblastoma

Yuan (Verified Customer) (01-23-2020)

The antibody is ok. Signal is not very strong for western blot. Needed relative long exposure for Hela cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hela cell

Kyosuke (Verified Customer) (06-12-2019)

I use this as a proliferation marker and this antibody works very well for WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: rat aortic smooth muscle cells