CCT2 Monoclonal antibody, PBS Only

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  WB analysis using 68214-1-Ig (same clone as 68214-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 68214-1-Ig (same clone as 68214-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68214-1-PBS in a different storage buffer formulation.

CCT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68214-1-PBS

Clone No.2H6C7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

99D8.1, CCT beta, CCTB, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2

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Product Information

68214-1-PBS targets CCT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19264

  Product name: Recombinant human CCT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 186-535 aa of BC113516

  Sequence: AVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

• Full Name: chaperonin containing TCP1, subunit 2 (beta)
• Calculated Molecular Weight: 535 aa, 57 kDa
• Observed Molecular Weight: 50-58 kDa
• GenBank Accession Number: BC113516
• Gene Symbol: CCT2
• Gene ID (NCBI): 10576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P78371
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with the molecular mass of 53 and 57 kDa. (PMID: 35699934)