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CD100 Monoclonal antibody

CD100 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human and More (1)







Cat no : 66582-1-Ig


A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human lymphoma tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:225-1:900
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66582-1-Ig targets CD100 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD100 fusion protein Ag26461
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC054500
Gene Symbol SEMA4D/CD100
Gene ID (NCBI) 10507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.


Product Specific Protocols
WB protocol for CD100 antibody 66582-1-IgDownload protocol
IHC protocol for CD100 antibody 66582-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Oncol Lett

Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer

Authors - Xiang Liu