CoraLite®488-conjugated Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for FC, IF

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

IF, FC, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

N418

Cat no : CL488-65130

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418



Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65130 targets CD11c in IF, FC, ELISA applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Mouse spleen dendritic cells
Full Name integrin alpha X
GenBank accession numberBC167225
Gene symbol Itgax
Gene ID (NCBI) 16411
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Protocols

Product Specific Protocols
IF protocol for CL488 CD11c antibody CL488-65130Download protocol
FC protocol for CL488 CD11c antibody CL488-65130Download protocol
Standard Protocols
Click here to view our Standard Protocols