CoraLite® Plus 488 Anti-Mouse CD11c (N418)

• 

  IF Staining of mouse splenocytes using CL488-65130

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD11c antibody (CL488-65130, Clone: N418 ) at dilution of 1:400.

• 

  FC experiment of mouse splenocytes using CL488-65130

  1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11c (CL488-65130, Clone: N418) or 0.25 ug isotype control. Cells were not fixed.

CD11c Monoclonal Antibody for IF/ICC, FC

Cat No. CL488-65130

Clone No.N418

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

IF/ICC, FC

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

---

Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65130 targets CD11c in IF/ICC, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse spleen dendritic cells
• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: CD11c
• Gene ID (NCBI): 16411
• RRID: AB_2883193
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q9QXH4
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).