FITC Plus Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

Publications

3

CloneNo.

N418

Cat no : FITC-65130

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

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Validation Data Gallery

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  FC experiment of mouse splenocytes using FITC-65130

  1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse MHC Class II (I-A/I-E) (APC-65122, Clone: M5/114.15.2) and 0.25 ug FITC Plus Anti-Mouse CD11c (FITC-65130, Clone: N418) or 0.25 ug FITC Plus Hamster IgG isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

FITC-65130 targets CD11c in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse spleen dendritic cells
• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: Itgax
• Gene ID (NCBI): 16411
• RRID: AB_2883792
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Publications

Species	Application	Title
mouse	FC	J Mater Chem B; PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer; Authors - Lian Zhu
mouse	FC	Nat Commun; Bacterial outer membrane vesicle based versatile nanosystem boosts the efferocytosis blockade triggered tumor-specific immunity; Authors - Wan-Ru Zhuang
mouse	FC	Acta Biomater; Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment; Authors - Zhuo Cheng