Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65130 targets CD11c in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse spleen dendritic cells Predict reactive species |
| Full Name | integrin alpha X |
| GenBank Accession Number | BC167225 |
| Gene Symbol | CD11c |
| Gene ID (NCBI) | 16411 |
| RRID | AB_2883792 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q9QXH4 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD11c antibody FITC-65130 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FITC-65130 is an Anti-Mouse CD11c (N418) monoclonal antibody with 8 total citations. Citations appear in Nature Communications, Science Advances, Bioactive Materials, Acta Pharmaceutica Sinica B, Journal of Advanced Research, Acta Biomaterialia, Journal of Materials Chemistry B, and Advanced Healthcare Materials. Research fields span tumor immunotherapy, nanovaccine design, dendritic cell activation, ferroptosis-immunotherapy synergy, and periodontitis treatment, predominantly in mouse models using flow cytometry.
| Species | Application | Title |
|---|---|---|
Bioact Mater Turning foes to friends: Advanced "in situ nanovaccine" with dual immunoregulation for enhanced immunotherapy of metastatic triple-negative breast cancer | ||
Nat Commun Bacterial outer membrane vesicle based versatile nanosystem boosts the efferocytosis blockade triggered tumor-specific immunity | ||
J Adv Res Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis | ||
Acta Pharm Sin B Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy | ||
Sci Adv Intratumoral antigen-presenting cell activation by a nanovesicle for the concurrent tertiary lymphoid structure de novo neogenesis | ||
Acta Biomater Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment |
