Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65130 targets CD11c in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse spleen dendritic cells Predict reactive species |
| Full Name | integrin alpha X |
| GenBank Accession Number | BC167225 |
| Gene Symbol | CD11c |
| Gene ID (NCBI) | 16411 |
| RRID | AB_2883914 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q9QXH4 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD11c antibody PE-65130 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD11c (N418) (PE-65130) has 12 citations across journals including J Extracell Vesicles, Phytomedicine, Mater Today Bio, Adv Healthc Mater, Int J Biol Macromol, Int J Pharm, Colloids Surf B Biointerfaces, Nutrients, Bioorg Chem, and Mol Pharm. Research fields span nanomedicine and nanoparticle delivery, cancer immunotherapy (hepatocellular carcinoma, breast cancer, pyroptosis–STING axis), mucosal and systemic immunity, dendritic cell biology, and infectious disease treatment.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles High-Yield Outer Membrane Vesicles Derived From Probiotics as a Nanoplatform for Precise Treatment and Prophylaxis of Pseudomonas aeruginosa Infection. | ||
Phytomedicine Glycyrrhetinic acid orchestrates ERS-pyroptosis crosstalk to elicit immunogenic cell death in hepatocellular carcinoma. | ||
Adv Healthc Mater Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization | ||
Mater Today Bio An MRI-visible nanotheranostic establishes a self-amplifying pyroptosis-STING-IFN-β circuit for CD8(+) T cell immunoactivation. | ||
Mater Today Bio Self-assembly of paclitaxel derivative and fructose as a potent inducer of immunogenic cell death to enhance cancer immunotherapy | ||
Int J Biol Macromol The impact of novel oral adjuvant dendritic fibrous nano-silica coated with M cell homing peptide-modified chitosan on enhancing both the intestinal mucosal and systemic immunity. |
