CoraLite®488-conjugated Anti-Mouse CD127 (IL-7Ra) (A7R34)

CD127 (IL-7Ra) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

FC, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

A7R34

Cat no : CL488-65093

Synonyms

CD127, IL 7Ralpha, Il7r, interleukin 7 receptor



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65093 targets CD127 (IL-7Ra) in FC, ELISA applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-7Ra-IgG1 fusion protein
Full Name interleukin 7 receptor
GenBank accession numberBC089571
Gene symbol Il7r
Gene ID (NCBI) 16197
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

Protocols

Product Specific Protocols
FC protocol for CL488-conjugated CD127 (IL-7Ra) antibody CL488-65093Download protocol
Standard Protocols
Click here to view our Standard Protocols