CD138/Syndecan-1 Monoclonal antibody, PBS Only (Capture)

• 

  WB analysis of BxPC-3 using 60185-1-Ig (same clone as 60185-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60185-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60185-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50044-1

  Sandwich ELISA standard curve of MP50044-1, SDC1,CD138 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60185-1-PBS. Detection antibody: 67155-1-PBS. Standard: Ag17733. Range: 0.188-12 ng/mL.

• 

  Sample test of MP50044-1

  Plasma of sixteen individual healthy human donors was measured. The CD138/Syndecan-1 concentration of detected samples was determined to be 293.79 ng/mL with a range of 89.65-474.60 ng/mL.

CD138/Syndecan-1 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 60185-1-PBS

Clone No.1B6A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD138, 1B6A2, SDC, SDC1, SYND1

---

Product Information

60185-1-PBS targets CD138/Syndecan-1 as part of a matched antibody pair:

MP50044-1: 60185-1-PBS capture and 67155-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0823

  Product name: Recombinant human SDC1,CD138 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC008765

  Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA

• Full Name: syndecan 1
• Calculated Molecular Weight: 32.5 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC008765
• Gene Symbol: CD138
• Gene ID (NCBI): 6382
• RRID: AB_10859833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P18827
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.