CD138/Syndecan-1 Recombinant monoclonal antibody

• 

  WB analysis using 84069-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84069-6-RR (CD138/Syndecan-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD138/Syndecan-1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84069-6-RR

Clone No.241278A4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

241278A4

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84069-6-RR targets CD138/Syndecan-1 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: syndecan 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_011519.2
• Gene Symbol: Sdc1
• Gene ID (NCBI): 20969
• RRID: AB_3671637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P18828-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.