CD14 Monoclonal antibody
CD14 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, mouse and More (1)
WB, IHC, IF, FC, Cell treatment, ELISA
Cat no : 60253-1-Ig
Validation Data Gallery
|Positive WB detected in||THP-1 cells, RAW 264.7 cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||RAW 264.7 cells, human tonsillitis tissue|
|Positive FC detected in||THP-1 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 2 publications below|
|IHC||See 4 publications below|
|IF||See 3 publications below|
|FC||See 1 publications below|
The immunogen of 60253-1-Ig is CD14 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rabbit|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD14 fusion protein Ag10693|
|Full Name||CD14 molecule|
|Calculated molecular weight||375 aa, 40 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC010507|
|Gene ID (NCBI)||929|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
|Product Specific Protocols|
|WB protocol for CD14 antibody 60253-1-Ig||Download protocol|
|IHC protocol for CD14 antibody 60253-1-Ig||Download protocol|
|IF protocol for CD14 antibody 60253-1-Ig||Download protocol|
|FC protocol for CD14 antibody 60253-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sunil (Verified Customer) (05-15-2023)
Great and fast delivery which is always useful!
Emma (Verified Customer) (11-29-2021)
Works well by IF on FFPE tissue. Tris-EDTA antigen retrieval pH 9.0.