PE Anti-Human CD154 (24-31)

CD154 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

24-31

Cat no : PE-65156

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

PE-65156 targets CD154 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / Mouse IgG1
Class Monoclonal
Type Antibody
Immunogen human CD154 fusion protein
Full Name CD40 ligand
Calculated molecular weight 261 aa, 29 kDa
GenBank accession numberBC071754
Gene symbol CD40LG
Gene ID (NCBI) 959
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496, 565 nm/578 nm
Form Liquid
Purification Method The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

Protocols

Product Specific Protocols
FC protocol for PE CD154 antibody PE-65156Download protocol
Standard Protocols
Click here to view our Standard Protocols